ABSTRACT
Pyrazolo [1,5-a] pyrimidine derivatives has a possible antianxiety properties and anti-inflammatory actions. The two pyrazolo [1,5-a] pyrimidine derivatives; 3-acetyl-5,7-dimethyl pyrazolo [1,5-a] pyrimidine as compound A and 3-chloro-5,7 dimethyl pyrazolo [1,5-a] pyrimidine as compound B were examined to evaluate their clastogenic or mutagenic properties through the induction of chromosomal aberrations in the somatic [bone-marrow] and germ [1ry spermatocyte] cells of mice. Mice treated orally by gavage with either compound A or compound B with a single dose [1/8, 1/4 and 1/2 LD50] and multiple doses with 1/8 LD50 for five consecutive days. Both compounds A and B were found to produce a significant increase in chromosomal aberrations in somatic and germ cells except with the low dose of compound A [1/8 LD50]. This increase was dose-dependent in both types of cells. Multiple treated animals caused a significant increase 24 hours after three and five days of treatment. Concerning the sperm abnormalities, compound B induced a significant increase in the sperm-head abnormalities. Slight inhibition of sperm count and sperm motility occurred after treatment of mice with 1/8 LD50 of compound B. The results showed that compound A had a low clastogenic effect than compound B